EFFECTS OF ADIPONECTIN ON MARKERS OF ENDOTHELIAL ACTIVATION AND MARKERS OF INFLAMMATION IN HUMAN CORONARY ARTERY ENDOTHELIAL CELLS

Authors

  • N. A. Rosman Faculty of Sports Science and Coaching, Universiti Pendidikan Sultan Idris, Tg Malim, Perak
  • G. R. A. Froemming Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA
  • O. Effat Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA
  • H. Nawawi Centre for Pathology Diagnostic and Research Laboratories, Faculty of Medicine, Universiti Teknologi MARA
  • H. J. Singh Centre for Pathology Diagnostic and Research Laboratories, Faculty of Medicine, Universiti Teknologi MARA

DOI:

https://doi.org/10.4314/jfas.v9i6s.81

Keywords:

HCAEC, Adiponectin, ICAM-1, E-selectin, IL-6, PAI-1, COX-2, NFkB

Abstract

This study investigates the effect of adiponectin on endothelial activation and inflammatory marker secretion by human coronary artery endothelial cells (HCEAC) in vitro.  Methodology: HCAEC at the seventh passage were divided into two groups and incubated for 24 hours at 37° C and 5% CO2 as follows: Control, and adiponectin-treated (30 µg/ml adiponectin) groups.  Supernatants were analysed for ICAM-1, E-selectin, PAI-1 and IL-6 and COX-2 using ELISA.  RT-PCR was used to analyse gene expression of ICAM-1, E-selectin, PAI-, IL-6, COX-2, NFkBp50 and NFkBp65. Data were analysed using independent t-test.  Results: ICAM-1 and E-selectin level was significantly higher in leptin-adiponectin-treated groups (P<0.01).  Endothelial activation marker protein level have no significant difference in adiponectin group when compared to control group.mRNA expression showed significant increase in adiponectin group as compared to control. PAI-1 and COX-2 showed no significant increase in the level of protein in adiponectin group but IL-6 showed significant increased in the protein level (P<0.001) while mRNA expression of PAI-1 (P<0.05), COX-2 (P<0.01) and IL-6 (P<0.001) showed significant increase in treated group as compared to control.  mRNA expression also showed significant increase in both the NFkBp50 and NFkBp65 signalling pathway. Conclusion: Adiponectin increases the secretion of IL-6 from HCEAC.  This adipokines might have a significant role in the inflammatory and pro-atherogenic state of obesity.

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Published

2017-11-10

Issue

Section

Research Articles