DETERMINATION OF SERUM INTERLEUKIN 33 (IL-33) LEVELS IN ATOPIC ASTHMA PATIENTS USING ELISA KITS

Authors

  • N. S. M. A. Siti School of Diagnostic and Biomedicine, Universiti Sultan ZainalAbidin, Gong Badak Campus, 21300 Kuala Nerus, Terengganu
  • S. M. A. Noor Department of Immunology, School of Medical Sciences, UniversitiSains Malaysia, Health Campus, 16150 KubangKerian, Kelantan
  • A. R. Azriani Department of Community Medicine, School of Medical Sciences, UniversitiSains Malaysia, Health Campus, 16150 KubangKerian, Kelantan

DOI:

https://doi.org/10.4314/jfas.v9i6s.69

Keywords:

IL-33, atopic asthma, serum levels, severity, ELISA.

Abstract

This study compares serum IL-33 levels in atopic asthma (AA) patients and normal controls to determine serum IL-33 levels. 5 mL of blood were withdrawn from subjects and centrifuged for 5 min at 2000 rpm to obtain the serum and were analyzed for IL-33 using kits. Data entry and analysis were done using SPSS version 20.0. The results showed serum IL-33 levels were higher in atopic asthma patients than in normal controls. This difference was not statistically significantwith a p-value of 0.218 determined by an independent-T test. The results also showed no significant difference in IL-33 levels according to AA severity with a p-value 0.396 determined by a Kruskal Wallis test. There was no significant difference of serum IL-33 levels between normal controls and AA patients. The levels of IL-33 and other predisposing factors showed significant associations in smoking status and occupational exposure.

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Published

2017-11-10

Issue

Section

Research Articles