ANTIOXIDANT ACTIVITY OF HIGH-DENSITY LIPOPROTEIN (HDL) USING DIFFERENT IN VITRO ASSAYS

Authors

  • W. N. W. Yusoff Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor
  • N. A. A. Bakar Faculty of Medicine, UniversitiTeknologi MARA, 47000 Sungai Buloh, Selangor
  • S. Muid Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor
  • A. M. Ali Faculty of Bioresources and Food Industry, Universiti Sultan ZainalAbidin (UniSZA), 20400 Terengganu
  • G. R. A. Froemming Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor
  • H. Nawawi Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor

DOI:

https://doi.org/10.4314/jfas.v9i6s.24

Keywords:

high-density lipoprotein, reverse cholesterol transport, apolipoprotein A1, antioxidant, in vitro.

Abstract

The role of HDL in reverse cholesterol transport is well known but other atheroprotective mechanism, e.g. antioxidant capacities of HDL is not clear. The aim of this study is to determine the effects of different doses of HDL on antioxidant activities assay. HDL was isolated from plasma by single step-ultracentrifugation. The antioxidant activity of different doses of HDL were measured by ferric thiocyanate (FTC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and dichlorofluorescein (DCF) tests. Coincubation of HDL with LDL showed longer lag time and lower reaction rate in a dose-dependent manner compared to LDL alone (p<0.05). HDL had inhibitory effects on radical oxygen species (ROS) production but did not exhibit free radical scavenging activities. HDL is a potent antioxidant in terms of inhibition of lipid peroxidation, ROS production and LDL oxidation. These may to some extent add to the antiatherogenic beyond reverse-cholesterol transport properties of HDL

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Published

2017-11-10

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Section

Research Articles